RCI-JNJN-70399 Oligonucleotide Analytics Scientist (siRNA)-San Diego, CA

Role is 100% onsite Scientist I, Oligonucleotide Analytics Specialized field of analytical characterization of siRNA using LC-MS methods San Diego, CA • Must be able to commute to work every day • PhD is required • Must have experience with Analytical Chemistry • Must have experience with Oligonucleotides • Must have siRNA experience • Previous gene therapy experience • Must have 1-3 years of experience • Experience with Chromatography (LC-MS) • Experience with these instruments: Thermo LTQ, LC/MS or Thermo Orbitrap LC/MS Key Responsibilities: • 30% Operating LC-MS systems to perform in-process and final QC of various scales of highly modified oligonucleotides, including siRNA and ASO, to support both discovery portfolio and pre-portfolio projects • 20% Optimize methods and improve analytical capabilities for novel Oligonucleotide synthesis • 10% • Analyze and optimize methods in a project-specific way (i.e. GalNAc vs Cholesterol vs C16) by denaturing and non-denaturing HPLC methods. In addition, phosphoramidites or lipids by HPLC and LCMS. • 20% Establish and automate high throughput analytical capabilities and coordinate those efforts with higher synthesis throughput. • 5% Analyze oligonucleotides by HPLC-SEC, RP-IP and Ion-Exchange Chromatography. • Continue optimizing siRNAs using best-in class analytical chemistry methodologies such as different column matrixes for optimal siRNA characterization • Support integrity confirmation requests (such as concentration) by assay development and biology teams • 3% Conduct QC for lipid nanoparticle (LNP) formulations of mRNA • 5% Ensure the supply of laboratory consumables and reagents are in stock as needed • Maintain a high level of productivity in the laboratory setting. • 2% Present data and reports on project status at individual, group, and departmental meetings. Qualifications • Ph.D.. with 1-3 years of experience, performing progressively advanced duties at the Scientist level, or the equivalent training/experience is required. • Prior experience in the characterization of oligonucleotides and/or nucleoside/nucleotide analytes is required. Top Three Skills: • Operate LC-MS systems - Analyze and optimize methods - establish and automate high throughout analytical capabilities