Scientist II NGS (Illumina MiSeq/iSeq) B‑cell Purification and Handling

100% ONSITE Pay: $48-$52/hr REQUIRED: • Minimum: Master’s degree + 2‑5 years relevant experience • Bachelor’s acceptable with 5 years strong hands‑on background • Background in microbiology, immunology, cell/molecular biology, or biotech • Hands‑on experience with NGS (Illumina MiSeq/iSeq) – must‑have • Microfluidics: emulsion, droplet generation, custom or commercial systems • B‑cell purification and handling • Molecular biology: polymerase reactions, RNA/DNA purification, indexing • Basic data analysis; familiarity with commercial sequencing software • Scripting in Python or R for data processing (plus) • Bioinformatics tools (IMGT, IgBLAST) • Protein detection methods (ELISA, similar assays) • Experience with other NGS platforms (Thermo Fisher, PacBio, ONT) – nice to have Job Description: B-Cell Sequencing is the focus of this group - downstream (Extracting antibodies from individual B cells and sequencing them to find new drug candidates) The successful candidate will support the development of new paired antibody sequencing technologies from single B cells using droplet microfluidics. Responsibilities include experimental planning and execution, comprehensive B cells sequencing analysis and presentation, and detailed documentation to support multiple programs across our in vivo antibody discovery portfolio. Responsibilities - Experimental Design & Execution: Independently design, execute and interpret experiments utilizing microfluidics to amplify heavy and light chain genes and generate DNA libraries for deep sequencing of single B cells captured in droplet emulsions. - Understand the broad objectives of the project as well as their role in achieving those objectives, and modify the experimental plan when required. Set and meet experimental timelines. - Molecular Techniques: Demonstrate a high level of proficiency in conducting RNA isolation, and DNA purification, and related methods to amplify antibody heavy- and light-chain variable regions from single cells or lysates and submit DNA libraries for next generation sequencing (MiSeq/MiSeq i100). - Bioinformatics Data Analysis: Perform comprehensive in silico antibody genetic analysis and data visualization using standard and custom antibody bioinformatic tools – to guide program decisions and deliver biotherapeutic candidates. - Cross-Functional Collaboration: Collaborate with multidisciplinary teams to present/discuss experimental findings to ensure alignment with overall biotherapeutic development goals. - Ability to work independently and as part of a team, managing multiple projects simultaneously.